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KMID : 1134820170460080919
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 8 p.919 ~ p.928
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Immuno-Enhancing Effect of Enzymatic Extract of Sargassum coreanum Using Crude Enzyme from Shewanella oneidensis PKA 1008
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Park Sun-Hee
Kim Min-Ji
Kim Go-Eun
Park So-Yeong
Kim Koth-Bong-Woo-Ri
Kim Yeon-Ji
Cho Young-Je
Ahn Dong-Hyun
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Abstract
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The immuno-enhancing effects of alginate oligosaccharides from Sargassum coreanum were investigated. The alginate oligosaccharides were produced by an alginate-degrading enzyme from S. oneidensis PKA 1008. The degraded alginate oligosaccharides were visualized by thin-layer chromatography developed using a solvent system of 1-butanol/methanol/water, 4:1:2 (v/v/v). Alginate was degraded into dimmers at 60 h. As a result, the levels of Th1 cytokine [interferon (IFN)-γ and interleukin (IL)-2] and Th2 cytokine (IL-6 and IL-10) increased with increasing incubation time compared to the control in vitro. Enzymatic extract treatment promoted proliferation of splenocytes at concentrations of 100 and 200 mg/kg at 24 h in vivo. Secretion of IFN-γ and IL-2 significantly increased in a dose-dependent manner at 24 h as well as induced higher production of IgG2a in serum. Natural killer cell activity was measured and tended to increase. In addition, complete blood cell counts increased in a dose-dependent manner. These results indicate that alginate oligosaccharides produced by crude enzyme from S. oneidensis PKA 1008 may have significant immune activities.
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KEYWORD
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Sargassum coreanum, Shewanella oneidensis PKA 1008, enzymatic extract, immuno-enhancing effects
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